Leicester City's Riyad Mahrez, Liverpool winger Sadio Mane and Manchester United central defender Eric Bailly are among 26 Premier League players called-up to take part.
The tournament, which is staged every two years, begins on January 14 and runs until February 5, meaning some players could miss up to four Premier League games, as well as cup games.
Sunderland, Leicester, Stoke and Watford have lost three players each to the tournament.
The clubs unaffected by the competition are Burnley, Chelsea, Manchester City, Middlesbrough, Swansea, Tottenham and West Brom. Liverpool defender Joel Matip will be available for selection, after refusing to join-up with Cameroon's squad.
Here's the full list of Premier League players who have departed for the Africa Cup of Nations:
Arsenal: Ismael Bennacer (Algeria), Mohamed Elneny (Egypt).
Bournemouth: Max Gradel (Ivory Coast).
Crystal Palace: Bakary Sako (Mali), Wilfried Zaha (Ivory Coast).
Everton: Idrissa Gueye (Senegal).
Hull City: Ahmed Elmohamady (Egypt), Dieumerci Mbokani (DR Congo).
Leicester City: Daniel Amartey (Ghana), Riyad Mahrez (Algeria), Islam Slimani (Algeria).
Liverpool: Sadio Mane (Senegal).
Manchester United: Eric Bailly (Ivory Coast).
Southampton: Sofiane Boufal (Morocco).
Stoke City: Wilfried Bony (Ivory Coast), Mame Biram Diouf (Senegal), Ramadan Sobhi (Egypt).
Sunderland: Wahbi Khazri (Tunisia), Lamine Kone (Ivory Coast), Didier N'dong (Gabon).
Watford: Nordin Amrabat (Morocco), Brice Dja Djedje (Ivory Coast), Adlene Guedioura (Algeria).
West Ham: Andre Ayew (Ghana), Cheikou Kouyate (Senegal).
West Brom: Allan Nyom (he is reporting for duty with Cameroon but has asked to withdraw and remain with the club.